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Differential Gene Expression Analysis Workshop (2023)

Note: In 2023, some workshops will be taught in person and others will be online via Zoom.

ONLINE Workshop dates and times:

TBD: 10 AM - 12 PM

TBD: 10 AM - 12 PM

TBD: 10 AM - 12 PM

TBD: 10 AM - 12 PM

Instruction will be mostly learner-centric, requiring a time commitment between the workshop sessions!


This hands-on workshop will introduce participants to statistical methods and considerations utilized to perform differential gene expression analysis on bulk RNA-seq data. Participants will learn about best practices in quality control, how to perform statistical analysis to obtain lists of differentially expressed (DE) genes using DESeq2. The workshop will also give participants an overview of tools for functional analysis of DE genes and how to extract some biological meaning from large gene lists.


This is one of our advanced workshops, and requires registants to have attended the following Basic workshop offered by our training team in the last 6-8 months:

I am fluent in R but have not attended the HBC prerequisite workshop, can I still register?

Yes, you can register AND please do the following:

Who should attend?

Eligible* Harvard researchers who have attended our Introduction to R workshop (or have working knowledge of R), and want to learn:


There is a non-refundable and non-transferable $65 registration fee for this advanced workshop.

We will be accepting 25 participants on a first-come, first-served basis:

NOTE: You will not have a reserved seat for this workshop until you pay the registration fee. Please make sure you pay within the time stated in that email, else you will lose your spot to someone on the waitlist.

*NOTE: HMS Graduate Students may receive waivers on registration fees. Please register and we’ll followup with you.


Please make sure you take the following into account when signing up for the workshop:

  1. Mandatory attendance for all four classes.
  2. 3-5 hours of reading and exercises from selected lessons before each workshop sessions.
  3. Active participation during discussion sessions.

Eligibility requirements:

*To be eligible to attend this workshop you should fulfill at least one of the following criteria:

If you are unsure of your eligibility, please register anyway and we will get back to you.

(Please check the eligibility requirements prior to registering)

NOTE: We do not record our training sessions.


Please email us at with any questions.