Set-up DESeq2 analysis - Answer Key
Exercise 1
Take a moment and answer the above questions for our VSM cells dataset.
Do you have enough cells to aggregate and do a pseudobulk analysis? Do you have enough power to run statistical tests?
Are there smaller cell states within a celltype that would be lost after aggregating with a pseudobulk approach?
Is there a latent variable that should be included in a design model?
Are there biological replicates that can be used to control for variability in the data?