FastQCFastQC Report
Wed 30 Sep 2015
Mov10_oe_1.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename Mov10_oe_1.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 39971841
Filtered Sequences 0
Sequence length 100
%GC 47

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 16795015 4.1748657 6.059911 2
CTGGG 8376590 2.5479658 6.4841547 1
TTCTT 12161990 2.543816 5.0711346 6
TCTTC 10938540 2.529536 5.185696 7
CTTCT 10885845 2.5173504 5.0255194 1
CTCCA 8804215 2.377327 8.132946 1
GGCAG 7360785 2.3646495 9.048611 1
TCCAG 8433860 2.3336692 5.7122536 7
CTCCT 8862290 2.2658334 6.7507205 1
CAGGA 7528755 2.2545867 5.9135337 1
CTTCA 9153500 2.235553 6.1804776 1
CCCAG 7216920 2.207828 6.119858 1
GCCAG 6455370 2.023714 6.2788043 1
CTGCA 7241750 2.0038092 5.1436768 1
CTTGG 6897085 1.8517264 5.505673 1
CTGGA 6511845 1.8464246 6.7122235 1
CTCAG 6449570 1.7846115 7.22948 1
CTTTT 8479045 1.7734871 5.9101095 1
TTTCA 7934210 1.7526736 5.187293 6
TTCAG 6830700 1.7095337 5.021524 7
CTTGA 5609765 1.4039677 5.2207584 1
CTCAT 4925100 1.2028538 5.1273108 1
CTCAA 4489260 1.1579475 5.334822 1